Host-Fungus Interactions

Contents

Part I: Gene Disruption 1. Gene Deletion in Candida albicans Wild-Type Strains Using the SAT1-Flipping Strategy Christoph Sasse and Joachim Morschhauser 2. Mini-Blaster-Mediated Targeted Gene Disruption and Marker Complementation in Candida albicans Shantanu Ganguly and Aaron P. Mitchell 3. Rapid Detection of Aneuploidy Following the Generation of Mutants in Candida albicans Megan D. Lenardon and Andre Nantel 4. Agrobacterium-Mediated Insertional Mutagenesis in Histoplasma capsulatumi Olga Zemska and Chad A. Rappleye 5. Targeted Gene Disruption in Cryptococcus neoformans Using Double Joint PCR with Split Dominant Selectable Markers Min Su Kim, Seo-Young Kim, Kwang-Woo Jung, and Yong-Sun Bahn 6. Multiple Gene Deletion in Cryptococcus neoformans Using the Cre--lox System Lorina G. Bakerand and Jennifer K. Lodge 7. Gene Disruption in Aspergillus fumigatus Using a PCR-Based Strategy and In Vivo Recombination in Yeast Iran Malavazi and Gustavo Henrique Goldman 8. Targeted Gene Deletion in Aspergillus fumigatus Using the Hygromycin-Resistance Split-Marker Approach Fabrice N. Gravelat, David S. Askew, and Donald C. Sheppard 9. Gene Disruption in Coccidioides Using Hygromycin or Phleomycin Resistance Markers Chiung-Yu Hung, Hua Zhang Wise, and Garry T. Cole Part II: Modulation of Gene Expression: RNAi Gene Knockdown 10. RNAi-Based Gene Silencing Using a GFP Sentinel System in Histoplasma capsulatum Brian H. Youseff and Chad A. Rappleye 11. RNA Interference in Cryptococcus neoformans Michael L. Skowyra and Tamara L. Doering 12. Gene Knock-Down in Paracoccidioides brasiliensis Using Antisense RNA Joao F. Menino, Agostinho J. Almeida, and Fernando Rodrigues Part III: Modulation of Gene Expression: Regulatable Promoters 13. Tetracycline-Inducible Gene Expression in Candida albicans Michael Weyler and Joachim Morschhauser 14. Galactose-Inducible Promoters in Cryptococcus neoformans var. grubii Lorina G. Baker and Jennifer K. Lodge 15. Modular Gene Over-Expression Strategies for Candida albicans Vitor Cabral, Murielle Chauvel, Arnaud Firon, Melanie Legrand, Audrey Nesseir, Sophie Bachellier-Bassi, Yogesh Chaudhari, Carol Munro, and Christophe d'Enfert Part IV: Responses to Infection In Vivo 16. Interactions Between Macrophages and Cell-Wall Oligosaccharides of Candida albicans Hector M. Mora-Montes, Christopher McKenzie, Judith M. Bain, Leanne E. Lewis, Lars P. Erwig, and Neil A.R. Gow 17. Murine Bone Marrow-Derived Dendritic Cells and T Cell Activation by Candida albicans Joanne Gibson, Neil A.R. Gow, and Simon Y.C. Wong 18. Phagocytosis and Intracellular Killing of Candida albicans by Murine Polymorphonuclear Neutrophils Alieke G. Vonk, Mihai G. Netea, and Bart Jan Kullberg 19. Human Oral Keratinocytes: A Model System to Analyze Host-Pathogen Interactions Torsten Wollert, Christiane Rollenhagen, George M. Langford, and Paula Sundstrom 20. Simple Assays for Measuring Innate Interactions with Fungi Ann M. Kerrigan, Maria da Gloria Teixeira de Sousa, and Gordon D. Brown 21. Binding and Uptake of Candida albicans by Human Monocyte-Derived Dendritic Cells Annemiek B. van Spriel and Alessandra Cambi 22. Immune Responses to Candida albicans in Models of In Vitro Reconstituted Human Oral Epithelium Jeanette Wagener, Daniela Mailander-Sanchez, and Martin Schaller 23. Analysis of Host-Cell Responses by Immunoblotting, ELISA, and Real-Time PCR David L. Moyes and Julian R. Naglik 24. In Vitro Model of Invasive Pulmonary Aspergillosis in the Human Alveolus Lea Gregson, William W. Hope, and Susan J. Howard 25. Biofilm Formation Studies in Microtiter Plate Format Marta Riera, Emilia Moreno-Ruiz, Sophie Goyard, Christophe d'Enfert, and Guilhem Janbon Part V: Responses to Infection In Vivo: Fungal Response 26. Transcript Profiling Using ESTs from Paracoccidioides brasiliensis in Models of Infection Alexandre Melo Bailao, Maristela Pereira, Silvia Maria Salem-Izacc, Clayton Luiz Borges, and Celia Maria de Almeida Soares 27. Laser Capture Microdissection of Candida albicans from Host Tissue Caroline Westwater and David A. Schofield 28. Isolation and Amplification of Fungal RNA for Microarray Analysis from Host Samples Anja Luttich, Sascha Brunke, and Bernhard Hube Part VI: Responses to Infection In Vivo: Host Response 29. Cytokine Measurement Using Cytometric Bead Arrays (CBA) Luis Castillo and Donna M. MacCallum 30. Transcript Profiling of the Murine Immune Response to Invasive Aspergillosis Zaneeta Dhesi, Susanne Herbst, and Darius Armstrong-James Part VII: Responses to Infection In Vivo: Model Systems for Host-Fungal Interactions 31. Caenorhabditis elegans: A Nematode Infection Model for Pathogenic Fungi Maged Muhammed, Jeffrey J. Coleman, and Eleftherios Mylonakis 32. Drosophila melanogaster as a Model Organism for Invasive Aspergillosis Michail S. Lionakis and Dimitrios P. Kontoyiannis 33. Galleria mellonella as a Model for Fungal Pathogenicity Testing John Fallon, Judy Kelly, and Kevin Kavanagh 34. Embryonated Chicken Eggs as Alternative Infection Model for Pathogenic Fungi Ilse D. Jacobsen, Katharina Grosse, and Bernhard Hube Part VIII: Responses to Infection In Vivo: Mammalian Hosts 35. Mouse Intravenous Challenge Models and Applications Donna M. MacCallum 36. A Nebulized Intra-Tracheal Rat Model of Invasive Pulmonary Aspergillosis Guillaume Desoubeaux and Jacques Chandenier 37. Invasive Models of Histoplasmosis A. George Smulian 38. Murine Model of Concurrent Oral and Vaginal Candida albicans Colonization Durdana Rahman, Mukesh Mistry, Selvam Thavaraj, Julian R. Naglik, and Stephen J. Challacombe 39. A Luciferase Reporter for Gene Expression Studies and Dynamic Imaging of Superficial Candida albicans Infections Donatella Pietrella, Brice Enjalbert, Ute Zeidler, Sadri Znaidi, Anna Rachini, Anna Vecchiarelli, and Christophe d'Enfert 40. Modeling of Fungal Biofilms Using a Rat Central-Vein Catheter Jeniel E. Nett, Karen Marchillo, and David R. Andes 41. Orogastrointestinal Model of Mucosal and Disseminated Candidiasis Karl V. Clemons and David A. Stevens 42. A Non-Lethal Murine Cutaneous Model of Invasive Aspergillosis Ronen Ben-Ami and Dimitrios Kontoyiannis