Guide to Protein Purification, 2nd Edition Volume 463

1. Why Purify Enzymes?
2. Strategies and Considerations for Protein Purifications (Linn)
3. Bioinformatics in Planning a Protein Purification (Burgess)
4. Preparing a Protein Purification Summary Table (Burgess)
5. Setting up a Laboratory (Deutscher)
6. Buffers: Principle and Practice (Blanchard)
7. Measurement of Enzyme Activity (Harris)
8. Quantitiation of Protein (Bailey and Noble)
9. Maintaining Protein Stability (Deutscher)
10. Strategy for Choosing and Expression System (Brondyk)
11. Bacterial Expression Systems (Collart)
12. Yeast Expression Systems (Cregg)
13. Baculovirus-Insect Cell Expression Systems (Jarvis)
14. Mammalian Expression Systems (Geisse and Knopf)
15. Expression of Tagged Proteins (Melhorta)
16. Re-folding of Solubilized Inclusion Body Proetin (Burgess)
17. Preparation of Bological Extract (Grabski)