Neuroprotection Methods and Protocols

Contents

TABLE OF CONTENTS 1) Neuronal death and neuroprotection (REVIEW CHAPTER) Mariaelena Repici, Jean Mariani and Tiziana Borsello 2) The use of propidium iodide to assess excitotoxic neuronal death in primary mixed cortical cultures. Anthony C Lau, Hong Cui, Michael Tymianski 3) A modified silver technique (De Olmos Stain) for assessment of neuronal and axonal degeneration. Tatyana I. Tenkova and Mark P. Goldberg 4) Model of acute injury to study neuroprotection. Michal Schwartz and Jonathan Kipnis 5) Organotypic entorhino-hippocampal slice cultures - a tool to study the molecular and cellular regulation of axonal regeneration and collateral sprouting in vitro. Domenico Del Turco and Thomas Deller 6) Role of Nrf2-dependent ARE-driven antioxidant pathway in neuroprotection. Jiang Li, Marcus J. Calkins, Delinda A. Johnson, Jeffrey A. Johnson 7) Biochemical methods to assess the coupling of brain energy metabolism in control and disease states. Jennifer Zechel, David W. Lust, Michele Puchowicz 8) A Method for Isolating Prosurvival Targets of NF-=B/Rel Transcription Factors. Francesca Zazzeroni, Christian Kuntzen, Can G. Pham, Salvatore Papa, Concetta Bubici, James R. Knabb, and Guido Franzoso 9) Functional cloning of genes regulating apoptosis in neuronal cells. Roberta Visconti and Luciano D'Adamio 10) Characterization of mRNA Expression in Single Neurons. David M. Lin, Brandon Loveall, John Ewer, David L. Deitcher, and Nikolaus J. Sucher 11) Brain on a chip: a method to detect novel neuroprotective candidate targets. Yang Tang and Myriam Bernaudin 12) Intracerebral infusion of neurotrophic factors. Theo Hagg 13) Synthesis of Cell Penetrating Peptides and Their Application in Neurobiology. Gunnar P.H. Dietz and Mathias Bahr 14) The stem cells as a potential treatment for neurodegeneration. Daniela Ferrari, Angelo Luigi Vescovi, Daniele Bottai 15) In Vivo Tomographic Neuroimaging Studies of Neurodegeneration and Neuroprotection (REVIEW CHAPTER) Rikki N. Waterhouse and Jun Zhao